Experimental Research on Effect of Medicated Serum contained with Yiqiyangyinjiedu Recipe on Proliferation and Apoptosis of Lewis Lung Cancer Cells in vitro

ObjectiveTo observe the effect of medicated serum contained with yiqiyangyinjiedu recipe on proliferation and apoptosis of Lewis lung cancer cells(LLC) in vitro. MethodsA total of 30 male rats waere divided into Low, medium, high dose Yiqiyangyinjiedu recipe, Cisplatin(DDP),Yiqiyangyin recipe combined with DDP and normal saline (NS) group. The medicated serum was prepared by the serum pharmacology method. Then the serum was added to LLC in vitro. The proliferation of LLC was measured by methyl thiazolyl tetrazolium(MTT) method. The cell apoptosis were measured by flow cytometry(FCM) and Annexin V- FITC/PI. Immunohistochemistry (SP method) measured the expression levels of Caspase-3. ResultsYiqiyangyin detoxification recipe containing drug serum had a remarkable growth-inhibition on LLC in vitro, these effects showed characters being time-dependent and concentration-dependent. Effect of high concentrations on LLC in 72h, the growth inhibiting rate(IR) was 50%. Inhibiting rate of yiqiyangyin detoxification recipe joint DDP containing drug serum up to 94.67%.Compared with the control group, each group containing serum can induce apoptosis of LLC, and showed characters being time-dependent and concentration-dependent. Yiqiyangyin detoxification recipe joint DDP containing drug serum on LLC for 72h, the apoptosis rate declined and the necrosis increase. Expression of Caspase-3 was being dose an time dependent manner(P<0.05). Compared with other groups, expression of Caspase-3 of chinese combined DDP was highest (P<0.05). Conclusion Yiqiyangyin detoxification recipe can inhibit proliferation and induce apoptosis of LLC. The promotion of Caspase-3 protein expression may be one of the mechanisms of inducing apoptosis of LLC.