Capsid Protein of Parvovirus B19 and COX-2 in Colon-rectal Adenocarcinomas

ObjectiveTo explore the relationship between the capsid protein of human parvovirus B19 and the COX-2 in colon-rectal adenocarcinoma. MethodsA total of 37 paraffin-embedded colon-rectal carcinoma tissues were analyzed by IHC for the expression of capsid protein and COX-2. The location of two kinds of proteins were investigated by double-staining of IHC. The method of Western blot was used to examine the expression of COX-2 and capsid protein. ResultsThere was statistical significance between capsid protein and COX-2 in goodness of fit. The expression of COX-2 was located in intracytoplasm whereas the capsid protein is in nucleus. Western blot showed the expression of COX-2 was up-regulated significantly in the cells transfected by VP1u compared to the controls transfected by pcDNA3.1. ConclusionThese results indicate a possible role of B19 in pathogenesis of colon-rectal adenocarcinoma by VP1u up-regulating the expression of COX-2.