Immunological Effects of Exosomes from Colorectal Cancer Cells Induced by Shocked Heat

ObjectiveTo investigate the effect of heat shock exosome (H-exo) from colorectal cancer cell on the protein factor secreted by the induced dendritic cells(DC) and the DC stimulating T-cell toxicity. Methods Colorectal tubular adenocarcinoma specimens through the clinical pathology diagnosis were isolated and cultured. After 1 hour heat shock at 43℃, the exosomes was obtained by four-step centrifugation and used to induce DC. The induced DC stimulates cytotoxic T lymphocytes for 24 hours. The production of TNF-α，MIP-1α，RANTES by DC was assessed by ELISA assay.By the MTT method T-cell toxicity were evaluated. Results We found that the heat shock exosomes (H-Exo) promoted DC to secrete TNF-α, MIP-1α, RANTES, significant differences compared with control group,P<0.05. To compare with the non-heat shock exosomes (Exo) group,The H-Exo induced cytotoxicity,P<0.05, and with the colorectal cancer cell lysate (Lys)group, was significantly different (P<0.01). To compare with the Exo and Lys group, there was significant difference (P<0.05),and the inductive effect of 100 μg/ml dose groups was stronger than 50 μg/ml. ConclusionHeat shock of colorectal cancer cells in culture can enhance immunological effects of exosomes with relation to inductive dose.