Cytotoxicity Effect of Thermo-chemotherpy with Pegylated Liposomal Doxorubicin on Human Esophageal Carcinoma in vitro

ObjectiveTo observe the cytotoxicity effect of thermo-chemotherapy with pegylated liposomal doxorubicin (PLD) on human esophageal squamous carcinoma cell line (EC9706). MethodsThe EC9706 cell line was treated, and cytotoxicity effect was observed with WST-1 assay, the free doxorubicin(ADM), and no drug group was positive control and negative control, respectively. Firstly, the 50% inhibiting concentration(IC50) of PLD and ADM was measured. Then using the IC50, the thermo-chemotherapy effect was measured after 37℃，41℃，43℃ 1 hour by a water bath heating system, respectively. ResultsThe average IC50 of ADM and PLD on EC9706 cell line was 19.064 μg/ml and 51.359 μg/ml(containing doxorubicin was 5.707 μg/ml), respectively. On 37℃, the cytotoxicity effect of ADM and PLD on EC9706 was (49.29±2.14)% and (49.39±6.07)%, respectively. There was no statistical significance(P>0.05); on 41℃, the effect of hyperthermia alone, ADM and PLD was (25.25±2.52)%，(64.80±5.01)% and (76.39±3.42)%，respectively. The showed the effect of the two drug was superior to hyperthermia alone(P<0.01), and PLD was superior to ADM(P<0.01), enhanced 11.59%, on 43℃, the effect of PLD (79.05±3.09)% was superior to ADM (71.89±2.30%)(P<0.05) which enhanced 7.16%, but in comparison with 41℃of PLD which enhanced only 2.66%.There was no statistical significance (P>0.05). ConclusionADM and PLD on EC9706 is superior to hyperthermia alone; on 37℃, the cytotoxicity effect of PLD is equal to ADM, on 41℃ and 43℃, the effect of PLD is superior to ADM, but the effect of PLD on 41℃ is equal to that on 43℃.