Effect of Agkihpin-an Arginine Esterase from Venom on Expression of MRP1 in CNE-2 Cells

ObjectiveTo explore the effect of Agkihpin on the expression of multidrug resistance associated protein 1(MRP1) in nasopharyngeal carcinoma CNE-2 cell line, and try to describe the mechanism of Agkihpin inhibiting CNE-2. MethodsThe cultured cells were treated with different concentrations of Agkihpin for 72 h. The expression level of MRP1 in CNE-2 cell line was assayed by immunocytochemistry, Western blot and RT-PCR. ResultsComparing with Agkihpin absent group, the MRP1 expression levels in CNE-2 cells were reduced significantly after the treatment of different concentrations of Agkihpin (P<0.05), in a concentration-dependent manner, which shows that Agkihpin could significantly down-regulate the expression of MRP1 in CNE-2 cells. ConclusionAgkihpin can inhibit the expression of MRP1 in CNE-2 cells, which is likely to be one of reasons for Agkihpin inhibiting the cellular vitality of nasopharyngeal carcinoma cell, and be likely to enhance the sensitivity of CNE-2 cells to chemotherapeutic drugs to some extent.