Apoptosis of Colon Cancer Cell Line HCT116 Induced by Flavone Components from Spina Gleditsiae

ObjectiveTo study the apoptosis of HCT116 Colon cancer cell line induced by Flavone Components from Spina Gleditsiae. MethodsThe inhibition of cell proliferation was determined by CCK-8(Cell Counting Kit-8) assay. The morphological changes of the apoptosis cells were observed by optical electron microscope after Hoechst33258 fluorescent staining and transmission electron microscope. The apoptosis rate of HCT116 cells was observed by Flow Cytometry with Annexin-V/PI donble staining. ResultsFlavone Components from Spina Gleditsiae significantly inhibited the proliferation of HCT116 cells in a dose-dependent manner with IC50 of (104.72±0.96) mg /L. Colon cancer cells apoptosis was morphologically observed after were 48h induced by Flavone Components from Spina Gleditsiae. The apoptosis of HCT116 cells was induced by different of Flavone Components from Spina Gleditsiae and the apoptosis rate was（35.00±3.76）% at 100 mg/L. ConclusionFlavone Components from Spina Gleditsiae can induce the apoptosis of HCT116 cells and inhibit their proliferation.