Effect of Low-dose Metronomic Chemotherapy on Growth Rate and VE-Cadherin of A549 Pulmonary Adenocarcinoma in Athymic Mice

ObjectiveTo investigate the growth rate and the expression of VE-Cadherin of tumor graft of the human pulmonary adenocarcinoma in nude mouse after chemotherapy with different dose of CTX. Methods Eighteen nude mice were subcutanously injected under the anterior right leg with cultured A549 cell suspend fluid. All the nude mice were devided into 3 groups. They were separately accepted the treatment of LDM CTX,MTD CTX and 0.9%NS.The volume of tumors were measured every week by sliding caliper. All nude mice were killed on the 21th day after treatment, then the tumors were taken out. Immunohistochemistry (Strepatacidin peroxidase System for Immunohistological Staining)was used to detect the expressions of VE-Cadherin and RT-PCR was used to detect the content of VE-Cadherin gene in the tumor tissues from 18 cases of nude mice. Results The growth rate, expressions of VE-Cadherin and content of VE-Cadherin gene in LDM group were significantly lower than that either in MTD group or in control group (P<0.05). Conclusion The Low-Dose Metronomic chemotherapy can down-regulate the level of VE-Cadherin and suppress the angiogenesis of tumor consequently then suppress the growth of tumor.