Association of Mutations in Human Papillomavirus Type 16 E7 and E5 Genes with Cervical Lesion in Hubei

ObjectiveTo identify variations in HPV 16 E7 and E5 genes and examine the association between these variants and cervical cancer development in Hubei,China. MethodsPolymerase chain reaction (PCR) and PCR-directed sequencing methods were applied.Samples from 115 women infected with HPV16 E5 and E7 were analyzed. ResultsIn addition to the prototype E7 and E5 genes sequence ，the most frequently combined mutations A647G and T846C of HPV16 E7 were detected 25% in normal cervix, 30% in CIN Ⅰ~Ⅱ,53.3% in CINⅢ and 62.9% in ICC, respectively. The E5 combined mutations of A3979C and A4042 were 30% in normal cervix, 33.3% in CINⅠ~Ⅱ、56.7%in CINⅢ and 71.4% in ICC, respectively. The combined mutations of HPV16 E7 and E5 were statistically different at different stages of cevercal lesion. ConclusionThe most prevalent variations were HPV 16E7 A647G- E7 T846C - E 5 A4042G - E5A3979C in Hubei, China.This combined variation was positively associated with cervical disease development.