Enhancing Sensibility of Ovarian Cancer Cell Line SKOV3 to Paclitaxel by Inhibiting Gene GSK-3β with RNA Interference

ObjectiveTo consrtuct the specific short hairpin interfering vector of gene GSK-3β and study its suppressing effect to understand mechanisms of enhancing sensibility of ovarian cancer cell line SKOV3 to taxol. MethodsDesign and construct specific short hairpin interfering vector of gene GSK-3β and transfect it into SKOV3 cell line for screening stable cell lines. Detect RNAi suppressing efficacy using RT-PCR and Western blot.Compare the different apoptosis ratio and cell sensibility to taxol before and after interfering gene GSK-3β by FACS. ResultsThe interfering vector pGSK3β-shRNA was constructed successfully.The expression of mRNA and protein of gene GSK-3β in stable cell line transfected with vector pGSK3β-shRNA was obviously lower than that in cells before transfected by RT-PCR and Western blot.Moreover, FACS results demonstrated the apoptosis ratio after 24h treatment of taxol was obviously increased and sensitivity was better in cells after transfected than before with significant difference(P<0.05). ConclusionThe constructed vector pGSK3β-shRNA had a specific and high effective inhibiting effect on gene GSK-3β. It could enhance the sensibility of ovarian cancer cell line SKOV3 to taxol and promote tumor cell apoptosis by inhibiting gene GSK-3β.