Downregulation of Expression of Moesin in U251 Cells after Transfection with siRNA Leads PDGF and CD44 to Decrease

ObjectiveTo explore the possible ways how Moeisn proteins involved in the growth and invasion of human brain glioblastoma (U251). Methods The human brain glioblastoma cell line U251 was chosen as the research object.Small interfering RNA fragment was designed for Moesin coding gene and transfected into cells.Through the reverse transcription polymerase chain reaction (RT-PCR) was screen out the most efficient siRNA silencing fragments for follow-up experiments.Three different intervention U251 cells (blank control group，negative control and transfection group) were designed for RT-PCR determination of expression of PDGF，and the amount of CD44 was measured by flow cytometry. Results Screening of a fragment of Moesin-139 by RT-PCR and Western-blot was the best silent; RT-PCR found that PDGF mRNA expression of U251 cells in the transfected group was 0.09377±0.008546 on average which was much lower than that of the blank group (0.4663±0.01844) and negative control group was (0.4570±0.02159).The difference was of obviously statistical significance (P<0.01).Flow cytometry detected that the expression of CD44 in transfected group was (26.73±2.720)% at minimum which had obvious differences with 2 other groups (F=173.669，sig=0.000 ). Conclusion In U251 cells，the downregulation of the expression of Moesin will lead to PDGF and CD44 to decrease at the same time.Moesin probably plays an important role in the invasion and metastasis of in human glioma cell by regulating PDGF and CD44.Moesin may become a target molecule in the treatment of human glioma.