Effects of Qxaliplatin Inhibition of Tumor Growth, Invasion and Metastasis Properties of Human Hepatocarcinoma Cells in vitro

Abstract:Objective To investigate the effects of oxaliplatin on inhibiting the proliferation,apoptosis, adhesion,and invasion of human Hepatocarcinoma cells (HepG2) in vitro. Methods MTT assay and AnnexinV/PI flow cytometry were used to examine the effect of oxaliplatin on the proliferation and apoptosis of the HepG2 cells, respectively. The adhesion of the cells was measured by MTT assay. The migration and invasion ability were tested with Transwell (Boyden chamber) and matrigel, respectively. Results The proliferation of the HepG2 cells was inhibited by oxaliplatin in the concentration of （10.0～40.0） mg/L and in a dose and time-dependent manners (P<0.01). Oxaliplatin in the concentration of 2.5 and 5.0 mg/L had no obvious effects on the incidence of proliferation. The number of apoptosis of HepG2 cells treated with oxaliplatin in the concentration of （2.5～10.0） mg/L for 24h was increased (P<0.05). HepG2 cells co incubated with oxaliplatin in the concentration of 2.5 mg/L for 30,60,90 and 120 min showed higher cell adhesion than the control group(P<0.05).The adhesion inhibition ratios were 29.2%,27.3%,26.6% and 24.8%, respectively. In addition, the ratio of migration and invasion for the HepG2 cells treated with oxaliplatin in the concentration of 2.5 mg/L for 24h was significantly reduced compared with that in the control groups(P<0.01). The migration and invasion inhibition rate were 53.68% and 54.38%, respectively. Conclusion Oxaliplatin may play an anti-metastatic role not only in inhibiting cells proliferation and induce apoptosis, but also in inhibiting the adhesion, migration and invasion of HepG2 cells in vitro.