Mechanism of Inhibition of Thermo-Chemotherapy on Cell Proliferation of Human Small Cell Lung Cancer

Abstract:Objective To study the inhibition of thermo-chemotherapy on lung cancer and its possible mechanism. Methods H446 cells were subjected to different thermo-chemotherapy strategies： 43 ℃+Paclitaxel (120 μg/L), 43 ℃+Paclitaxel(120 μg/L)+SP600125(20 μmol/L, JNK inhibitor), and Paclitaxel (120 μg/L).The untreated cells were served as control. MTT assay was used to measure cell proliferation and Western blot was used to examine the phosphorylation of JNK and the expression of Caspase-3. Results The proliferation rate of cells in the thermo-chemotherapy group was significantly lower than those in the other groups (P<0.05). The phosphorylation of JNK significantly increased in the thermo-chemotherapy group (P<0.05); SP600125 inhibited the phosphorylation of JNK and the proliferation of cells in the SP600125 group elevated (P<0.05). The expression of Caspase-3 in the thermo-chemotherapy group increased,which could be inhibited by SP600125 (P<0.05). The rate of apoptosis increased in the thermo-chemotherapy group(P<0.05) and decreased in SP600125 group (P<0.05). Conclusion Thermotherapy can promote the inhibition effect of Paclitaxel chemotherapy against the growth of lung cancer cell line H446, probably through activating JNK pathway and Caspase--3 to induce apoptosis.