Expression and Significance of AR and NF-κBp65 in Triple Negative Breast Carcinoma

Abstract: Objective To study the effect of rotary magnetic field (RMF) combining 5-Fu on the cycle and apoptosis of mouse cell line SP2/0 in vitro. Methods SP2/0 cells were randomly divided into four groups: control group (N), 5-Fu group (C), magnetic group (M) and magnetic combining 5-Fu group (M+C).The M and M+C groups were treated with a RMF for two hours once a day.On day 4, the C and M+C groups were treated with 5-Fu 20 μg/ml.On day 5, cell cycle and apoptosis were measured by the flow cytometric (FCM). Results The S phase proportion of the M group and the G1 phase proportion of the C group were higher than that of the other three groups（P<0.05）.The S phase proportion of the M+C group decreased and lower than that of the M group，but was still higher than that of the N and C groups（P<0.05）.There was no significant difference in apoptosis rates between the N and M groups（P>0.05）.The apoptosis rates of the C and M+C groups were remarkedly higher than those of the N and M groups and the M+C group had the highest apoptosis rate. Conclusion The RMF can't induce the apoptosis.But it can enhance the cytotoxicity of 5-Fu and promote the cell apoptosis.The mechanism of the apoptosis may be related to SP2/0 cell line arrested at S phase.Objective To investigate the expression and significance of AR and NF-ΚBp65 in triple negative breast carcinoma(TNBC). Methods The expression of AR and NF-ΚBp65 in 60 cases of TNBC were detected by immunohistochemical staining. Results The positive rate of AR was 35% (21/60), and that of NF-ΚBp65 was 70% (42/60), and there was no correlation between them.Compared with clinicopathologic features, AR-positive cases were more likely at lower tumor grade and had less mitosis count(P=0.026, P=0.032, respectively), but NF-κB p65-positive cases had more mitosis count(P=0.037).However,there was no significant difference found between their expressions and the others features. Conclusion AR and NF-κB have high positive rates in TNBC, so they may play critical roles in tumorigenesis of TNBC, and could be new treatment targets.