Expression of C-myc and HPV16/18 in Cervical Carcinoma and Cervical Intraepithelial Neoplasia and Their Relationship

Abstract: Objective To study the effect of rotary magnetic field (RMF) combining 5-Fu on the cycle and apoptosis of mouse cell line SP2/0 in vitro. Methods SP2/0 cells were randomly divided into four groups: control group (N), 5-Fu group (C), magnetic group (M) and magnetic combining 5-Fu group (M+C).The M and M+C groups were treated with a RMF for two hours once a day.On day 4, the C and M+C groups were treated with 5-Fu 20 μg/ml.On day 5, cell cycle and apoptosis were measured by the flow cytometric (FCM). Results The S phase proportion of the M group and the G1 phase proportion of the C group were higher than that of the other three groups（P<0.05）.The S phase proportion of the M+C group decreased and lower than that of the M group，but was still higher than that of the N and C groups（P<0.05）.There was no significant difference in apoptosis rates between the N and M groups（P>0.05）.The apoptosis rates of the C and M+C groups were remarkedly higher than those of the N and M groups and the M+C group had the highest apoptosis rate. Conclusion The RMF can't induce the apoptosis.But it can enhance the cytotoxicity of 5-Fu and promote the cell apoptosis.The mechanism of the apoptosis may be related to SP2/0 cell line arrested at S phase.Objective To investigate the expression of C-myc and HPV16/18 DNA in cervical carcinoma and cervical intraepithelial neoplasia (CIN) and their relationship with clinicopathology. Methods 10 cases of normal cervical epithelium,20 cases of cervical intraepithelial neoplasia (CIN) and 30 cases of cervical carcinoma were included.The expression of C-myc gene was evaluated by immunohistochemistry (Maxvision).The expression of HPV16/18 DNA was determined by hybridization in situ. Results （1）The positive expression rate of C-myc was 20%（2/10） in normal cervical epithelium, 45%（9/20） in CIN and 66.7 %（20/30 ） in cervical carcinoma respectively.The positive expression rate of HPV16/18 DNA was 10%（1/10） in normal,50%（10/20） in CIN and 73.3 %（22/30 ） in cervical carcinoma respectively.Their difference were all significant(P<0.0 5).（2）The positive expression rate of C-myc elevated with FIGO, histological grade and the lymph node metastasis.Their difference was significant(P<0.0 5).（3）HPV16/18DNA was positively correlated with C-myc in cervical carcinoma. Conclusion (1) High-risk HPV was positively correlated with C-myc in cervical carcinoma, supportting that C-myc gene is a target gene of high-risk HPV.（2）HPV had the vital clinical significance of disease-screen and early diagnosis.（3）C-myc had important value to appraisal of the cervical carcinoma treatment result and estimate of the prognostic.