^{Expression and Significance of OPN in Stage ⅠA~ⅡA Cervical Carcinoma}

Objective To study the effect of rotary magnetic field (RMF) combining 5-Fu on the cycle and apoptosis of mouse cell line SP2/0 in vitro. Methods SP2/0 cells were randomly divided into four groups: control group (N), 5-Fu group (C), magnetic group (M) and magnetic combining 5-Fu group (M+C).The M and M+C groups were treated with a RMF for two hours once a day.On day 4, the C and M+C groups were treated with 5-Fu 20 μg/ml.On day 5, cell cycle and apoptosis were measured by the flow cytometric (FCM). Results The S phase proportion of the M group and the G1 phase proportion of the C group were higher than that of the other three groups（P<0.05）.The S phase proportion of the M+C group decreased and lower than that of the M group，but was still higher than that of the N and C groups（P<0.05）.There was no significant difference in apoptosis rates between the N and M groups（P>0.05）.The apoptosis rates of the C and M+C groups were remarkedly higher than those of the N and M groups and the M+C group had the highest apoptosis rate. Conclusion The RMF can't induce the apoptosis.But it can enhance the cytotoxicity of 5-Fu and promote the cell apoptosis.The mechanism of the apoptosis may be related to SP2/0 cell line arrested at S phase.Objective To investigate the expression levels of osteopontin (OPN) in stage ⅠA~ⅡA cervical carcinoma and to study the pathological characteristics.To discuss their contribution to lympho-metastasis. Methods The expression of OPN was detected in 44 samples of cervical carcinoma with stage ⅠA~ⅡA and 15 samples of normal cervix by using SP method (streptavidin-perosidase，SP) to analyze the correlation with clinical pathological characters, lympho-metastasis and their clinical implication．Results (1)The positive expression rates of OPN was 72.73%(32/44) and 13.33% (2/15) in cervical carcinoma and normal cervix respectively with significant difference（P<0.05）.(2) The positive expression rates of OPN had no significant in cervical cancer's pathological grades, tissue types, invasive depth and ages（P>0.05）．But the expression had significant differences in stage Ⅰa, Ⅰb and stage Ⅱa（P<0.05）．OPN expression in patients with less than or equal one positive lymphonode was different from others with more than one positive lymphonodes（P<0.05）. Conclusion The positive expression rates of OPN in cervical carcinoma were higher and has significant differences in clinical stages．The positive expression of OPN corelates to amount of positive lymph nodes． The expression of OPN may be helpful to predicte lympho-metstasis．