Analysis of Virological Characteristics on HBeAg Positive Primary Hepatic Carcinoma

Abstract: Objective To study the effect of rotary magnetic field (RMF) combining 5-Fu on the cycle and apoptosis of mouse cell line SP2/0 in vitro. Methods SP2/0 cells were randomly divided into four groups: control group (N), 5-Fu group (C), magnetic group (M) and magnetic combining 5-Fu group (M+C).The M and M+C groups were treated with a RMF for two hours once a day.On day 4, the C and M+C groups were treated with 5-Fu 20 μg/ml.On day 5, cell cycle and apoptosis were measured by the flow cytometric (FCM). Results The S phase proportion of the M group and the G1 phase proportion of the C group were higher than that of the other three groups（P<0.05）.The S phase proportion of the M+C group decreased and lower than that of the M group，but was still higher than that of the N and C groups（P<0.05）.There was no significant difference in apoptosis rates between the N and M groups（P>0.05）.The apoptosis rates of the C and M+C groups were remarkedly higher than those of the N and M groups and the M+C group had the highest apoptosis rate. Conclusion The RMF can't induce the apoptosis.But it can enhance the cytotoxicity of 5-Fu and promote the cell apoptosis.The mechanism of the apoptosis may be related to SP2/0 cell line arrested at S phase.Objective To evaluate the relationship between positivity of HBeAg and the development of primary hepatic cancer. Methods A total of 1028 in-patient PHC cases were retrospectively analyzed.Portal vein tumor thrombus,serum AFP values, HBV DNA levels Child-Paugh Grading and histologic alterations were analyzed in both HBeAg-positive group and HBeAg-negative group. Results Of the 1028 cases, 895(87.1%) were HBeAg-negative and 133(12.9%) were HBeAg-positive.Compared with HBeAg-negative group, HBeAg-positive group had a significantly higher Portal vein tumor thrombus rate(χ2=4.46，P=0.035), significantly higher DNA levels(χ2=12.81，P=0.00).Compared with HBeAg-negative group, HBeAg-positive group had a significantly higher Child-Paugh Grading B and C(χ2=82.97，P=0.00).And the cases of hepatic fibrosis stage 4 had also higher in the HBeAg-positive group than in the HBeAg-negative group.No obvious difference in AFP levels was observed between these two groups. Conclusion There was more serious hepatic tissue injury in the HBeAg-positive group than in the HBeAg-negative group, and HBeAg-positive group had greater tendency of developing Portal vein tumor thrombus and liver cirrhosis with poor prognosis.