Expressions of Survivin,NFκB,VEGF-C and Their Relationship with MVD,MLVD in Small Cell Carcinoma of Esophagus

Abstract: Objective To study the effect of rotary magnetic field (RMF) combining 5-Fu on the cycle and apoptosis of mouse cell line SP2/0 in vitro. Methods SP2/0 cells were randomly divided into four groups: control group (N), 5-Fu group (C), magnetic group (M) and magnetic combining 5-Fu group (M+C).The M and M+C groups were treated with a RMF for two hours once a day.On day 4, the C and M+C groups were treated with 5-Fu 20 μg/ml.On day 5, cell cycle and apoptosis were measured by the flow cytometric (FCM). Results The S phase proportion of the M group and the G1 phase proportion of the C group were higher than that of the other three groups（P<0.05）.The S phase proportion of the M+C group decreased and lower than that of the M group，but was still higher than that of the N and C groups（P<0.05）.There was no significant difference in apoptosis rates between the N and M groups（P>0.05）.The apoptosis rates of the C and M+C groups were remarkedly higher than those of the N and M groups and the M+C group had the highest apoptosis rate. Conclusion The RMF can't induce the apoptosis.But it can enhance the cytotoxicity of 5-Fu and promote the cell apoptosis.The mechanism of the apoptosis may be related to SP2/0 cell line arrested at S phase.Objective To investigate the expression of Survivin,NF-κB,VEGF-C,MVD and MLVD, their relationships with the behaviors of the small cell carcinoma of the esophagus and with MVD and MLVD. Methods Envision immunohistochemical method was used to detect the expression of Survivin,NF-κB,VEGF-C and MVD,MLVD in 34 cases of the small cell carcinoma of the esophagus. Results Positive rates of survivin,NF-κB and VEGF-C positive rate was 35.29%,67.65% and 79.41%,respectively.There were no connection between the expression of Survivin,MLVD and the tumor's invasion depth and lymphatic metastasis.There was a significant correlation between the expression of VEGF-C,NF-κB,MVD and the tumor's lymphatic metastasis, but there were no relationship to the tumor's invasion depth.MVD in the positive expression of NF-κB was higher than those in the negative expressed cases. Conclusion There were significant correlations between VEGF-C,NF-κB,MVD and the tumor's lymphatic metastasis. Thus they may play an important role in the small cell carcinoma and may be a molecular biological basis to clinical therapy.