Primary Culture and Drug Sensitivity of Human Glioma Cells

Abstract: Objective To study the effect of rotary magnetic field (RMF) combining 5-Fu on the cycle and apoptosis of mouse cell line SP2/0 in vitro. Methods SP2/0 cells were randomly divided into four groups: control group (N), 5-Fu group (C), magnetic group (M) and magnetic combining 5-Fu group (M+C).The M and M+C groups were treated with a RMF for two hours once a day.On day 4, the C and M+C groups were treated with 5-Fu 20 μg/ml.On day 5, cell cycle and apoptosis were measured by the flow cytometric (FCM). Results The S phase proportion of the M group and the G1 phase proportion of the C group were higher than that of the other three groups（P<0.05）.The S phase proportion of the M+C group decreased and lower than that of the M group，but was still higher than that of the N and C groups（P<0.05）.There was no significant difference in apoptosis rates between the N and M groups（P>0.05）.The apoptosis rates of the C and M+C groups were remarkedly higher than those of the N and M groups and the M+C group had the highest apoptosis rate. Conclusion The RMF can't induce the apoptosis.But it can enhance the cytotoxicity of 5-Fu and promote the cell apoptosis.The mechanism of the apoptosis may be related to SP2/0 cell line arrested at S phase.ObjectiveTo establish the method of primary human glioma cells culture and to evaluate the sensitivity of different antitumor drugs on different glioma individuals. Methods The glioma cells from 39 glioma patients were cultured primarily by the tissue pieces culture method.To study the sensitivity of chemotherapeutic drugs of the glioma cells to five drugs, adriamycin(ADM), cis-dichlorodiamine platinum(DDP), vincristine (VCR), teniposide(VM-26) and 5-Floxuridine(5-Fu) were tested by MTT assay，and the results were compared. Results The primary human glioma cells were successfully cultured in 37 samples and 2 samples failed, with a successful rate 94.9 %.The success in 37 cases showed that diversity of inhibitory rates of antitumor drugs was observed in samples from different individuals.The rank of sensitivity from high to low is: VM-26>DDP>5-Fu>ADM>VCR.The effective rate of VM-26、 DDP、5-Fu、ADM and VCR was 56.8%、51.4%、37.8%、24.3% and 13.5% respectively. Conclusion It is feasible to primary culture glioma cells and to test chemotherapeutic drug sensitivity by MTT method.Determination of in vitro drug sensitivity of glioma patients with chemotherapy for the individual has a certain value.