Effect of Transfected Stem Cells with pCE Plasmid on Therapy for Liver Cancer

Abstract: ObjectiveTo study the effect of rotary magnetic field (RMF) combining 5-Fu on the cycle and apoptosis of mouse cell line SP2/0 in vitro. Methods SP2/0 cells were randomly divided into four groups: control group (N), 5-Fu group (C), magnetic group (M) and magnetic combining 5-Fu group (M+C).The M and M+C groups were treated with a RMF for two hours once a day.On day 4, the C and M+C groups were treated with 5-Fu 20 μg/ml.On day 5, cell cycle and apoptosis were measured by the flow cytometric (FCM). Results The S phase proportion of the M group and the G1 phase proportion of the C group were higher than that of the other three groups（P<0.05）.The S phase proportion of the M+C group decreased and lower than that of the M group，but was still higher than that of the N and C groups（P<0.05）.There was no significant difference in apoptosis rates between the N and M groups（P>0.05）.The apoptosis rates of the C and M+C groups were remarkedly higher than those of the N and M groups and the M+C group had the highest apoptosis rate. Conclusion The RMF can't induce the apoptosis.But it can enhance the cytotoxicity of 5-Fu and promote the cell apoptosis.The mechanism of the apoptosis may be related to SP2/0 cell line arrested at S phase.Objective To observe stem cells with pCE plasmid therapy for liver cancer, and explore its related mechanism. Methods After injection of stem cells into tail vein, inhibiting tumor ratio was calculated, satellite lesions in the liver, change of tumor mass in pathology, expression of SDF-1 were studied. The difference among control group, group of stem cells, group of stem cells with pCE plasmid, and group of 5-Fu were compared. Results Stem cells with pCE plasmid could inhibit proliferation of cancer cells (56%)in the following 10 day(P<0.05), compared with control group(0%), group of stem cells(8%) and group of 5-Fu(78%),respectively. Expression of SDF-1 from group of stem cells with pCE plasmid after treatment was increased (OD=0.48), different from control group(OD=0.35), group of stem cells(OD=0.39) and group of 5-Fu(OD=0.36) (P<0.05).Expression of SDF-1 from all groups before treatment did not have difference(P>0.05). Conclusion Stem cells with pCE plasmid could inhibit proliferation of cancer cells through the help of SDF-1.