Effects of GRIM-19 on Growth of Cervical Cancer Cell Xenograft Tumor

Abstract:Objective To explore the effects of GRIM-19 expression on the growth of cervical cancer in vivo and its mechanisms. Methods GRIM-19 gene expression was investigated at mRNA and protein levels by RT-PCR and Western blot.Tumor growth of the null mice was analyzed after injection HeLa/control,HeLa/GRIM-19 into the skin.The protein level of Ki-67 was inspected with immumohistochemistry methods. Results The growth of HeLa/GRIM-19 implanted tumor slowed down significantly（P<0.01）. The expression of GRIM-19 mRNA was up-regulated, while STAT3, cyclin B1, Bcl-L2 mRNA were decreased in tumors bearing HeLa/GRIM-19.The protein level of STAT3,p-STAT3,suvivin,ki-67 was reduced too; while the expression of GRIM-19 was increased by protein test. Conclusion The expression of GRIM-19 can slow down the proliferation of cervical cancer cells, suggesting that GRIM-19 may be a new target for the treatment of cervical cancer.