Effects of TRAIL on Proliferation,Apoptosis and NF-κＢ of Primary Human Ovarian Cancer Cell

Objective To investigate the regulatory effects of TRAIL on proliferation and apoptosis of primary human ovarian cancer cell in vitro，and the activation and transportation of NF-κＢ. Methods Primary human ovarian cancer cells were obtained from twelve patients.Each patient's cell was divided to six groups，and treated with TRAIL (5～100 μg/L) for 24～96 h respectively.The ratio of growth inhibition was measured with MTT in primary human ovarian cancer cells.The ratio of apoptosis and the transportation and activation of NF-κＢ were measured by immunofluorescence in primary human ovarian cancer cells. Results The ratio of growth inhibition increased in dose-and time-dependent manner when the concentration was 5，10 or 20 μg/L at 24,48,72h.The ratio of apoptosis increased along with the time lasting.It was (28±1.18)% with concentration of 100μg/L at 24h，and (55±4.12)% at 96h.The level of NF-κＢ in nucleus and cytoplasm was highly expressed. Conclusion TRAIL inhibits proliferation and growth of primary human ovarian cancer cell，and induces apoptosis.It enhances conveying the NF-κＢ from cytolymph to nucleolus.