Mechanism of Effect of p42/44MAPK Inhibitor U0126 on SGC-7901 Cell Proliferation and Apoptosis

Objective To study the effect of U0126 on cytobiological characteristics of SGC-7901. Methods To determine effect of U0126 on SGC-7901 cell proliferation by MTT.Flow cytometry was used to detect influence of U0126 on SGC-7901 cell cycle and apoptosis.The change of p42/44,p38,p-p42/44 and p-p38 was analyzed by Western blot. Results 10，15，20μM U0126 can restrain SGC-7901 cell proliferation，decrease S and G₂/M phase cell ratio and increase G₀/G₁ phase cell ratio.U0126 can induce tumor cell apoptosis and have a most powerful apoptosis in 20μM(P<0.01).U0126 can decrease p-p42/44 and increase p-p38. Conclusion U0126 can induce tumor cell apoptosis and inhibit cell proliferation.