Anti-proliferative and Anti-angiogenic Effects of Ginsenoside Rg3 on Human Nasopharyngeal Carcinoma HNE-1 Cell Line

Objective To study the inhibitory effects of Ginsenoside Rg3 on proliferation，migration and in vitro angiogenesis of human nasopharyngeal carcinoma HNE-1 cells. Methods The proliferative activity，migration and tube-like structures (TLSs) formation of HNE-1 cells，cultured in medium with different concentration of Ginsenoside Rg3，were determined by MTT assay，wound healing assay and in vitro anti-angiogenic test，respectively.Anti-angiogenic test was based on preliminary experiment on the observation of TLSs formed by HNE-1 cells on Matrigel and their structural characteristics. Results Ginsenoside Rg3 had anti-proliferative effect both in HNE-1 and CRL-2480 cells at the concentrations of 50，100 and 200μg/ml，which was dose-dependent but not time-dependent，and neither of them has statistical significance(P>0.05).Ginsenoside Rg3 could significantly decrease the migration velocity of HNE-1 cells at the concentrations of 25，50，100 and 200μg/ml，of which the differences were all statistically significant compared with control group(P<0.01)，and the effect was negatively correlated with the concentration of Ginsenoside Rg3 (r=-0.964；P<0.001).HNE-1 cells could form TLSs on matrigel.Ginsenoside Rg3 could inhibit the formation of TLSs of HNE-1 cells，and the number of tubule was negatively correlated with the concentration of Ginsenoside Rg3(r=-0.928； P<0.01). Conclusion Ginsenoside Rg3 has certain anti-proliferative effect on HNE-1 cells.HNE-1 cells could form TLSs on matrigel.Ginsenoside Rg3 can inhibit the migration and vasculogenic mimicry of HNE-1 cells.