Influence of Ad-EGFP-MDR1 on Mice Bone Marrow Cells

Objective：To study the cell cycle, apoptosis associated genes and proliferation of mice bone marrow cells (BMCs) infected by Ad-EGFP-MDR1 and to approach the genetic modified BMCs in somatic cell therapy. Methods：To assess colony formation ability of BMCs infected by adenovirus. Expression of Bax，Caspase-9 and p53 were measured by western-blot. Cell cycle was analyzed with flow cytometry. And trypan blue was used to evaluate cell viability. Results：The colony formation ability of BMCs infected by Ad-EGFP-MDR1 was not abnormal compare with the control group. No expression of Bax，Caspase-9 and p53 were detected in all of the cells. Cell cycle of each group was no change (P>0.05). Cells stained with trypan blue showed no significant statistic difference between the two groups (P>0.05). Conclusion ：Ad-EGFP-MDR1 has no obvious toxic effect on BMCs. It is useful to research the safety of gene therapy in vivo.