Establishment and Characteristics of VCR-resistant Cell Line (Raji/VCR) from Human Malignant Lymphoma Cell Line Raji

Objective：To establish VCR-resistant cell line (Raji/VCR) from human malignant lymphoma cell line Raji and to initially investigate its bionomics. Methods ：Pulse exposure to high dose VCR was used to establish resistant cell line Raji/VCR. MTT essay was used to study its drug sensitivity to VCR、VP-16 and ADM. LeveLs of DMR1 mRNA expression were detected by real time reverse transcription-polymerase chain reaction(real time RT-PCR). Intracellular rubidomycin(DNR) concentration and P-glycoprotein(P-gp) were examined by flow cytometry(FCM). Results ：The human multidrug-resistant malignant lymphoma cell line Raji/VCR was established after 8 months selective culture whose resistance multiple to VCR、ADM、VP-16 were 8，5，3 respectively.The expression of DMR1 mRNA and P-gp in Raji/VCR were significantly higher than that in Raji，but intracellular DNR concentration wgs markly reduced. Conclusion ：Raji/VCR have the basal bionomics of multidrug-resistant cell lines,and may be used for investigation related to multidrug-resistance of tumor.