Inhibitory Effect of Activated CD40Lig with Docetaxel on Breast Cancer Cell Proliferation

Objective：To explore the influence of rhCD40L with Docetaxel on proliferation of Breast Cancer Cells．Methods：The proliferation of breast cancer cell lines M231，M435 after treatment with rhCD40L and Docetaxel was analyzed by MTT and cell cycle distribution was detected by FCM with PI staining. The expression of CD54、CD95 and CD95L was also detected by FCM. Results：The proliferation of M231，M435 was inhibited after treatment with rhCD40L( P <0.05). The proliferation of M231，M435 wasfurther inhibited after treatment with rhCD40L and Docetaxel ( P <0.05).The cell number of stage G₁ and S stage was obviously increased，respectively( P <0.05),CD95 was increased obviously( P <0.05), but CD54 and CD95L was significantly decreased( P <0.05)．Conclusion：rhCD40L can enhance the effect of Docetaxel on proliferation inhibition of M231 and M435. It enhances the anti-tumor activity of Docetaxel by inducing cell G₁ arrest，up-regulating CD95 and down-regulating CD54，CD95L.