Screening of Upstream Regulators of Proto-Dbl Oncoprotein by Yeast Two-hybrid System

Objective：The VL domain truncate mutation could directly cause the activation of proto-Dbl and the malignant transformation. Methods：In order to clarify the activation mechanism of proto-Dbl, yeast two-hybrid system was used to screen the proteins interacted with the VL domain in the human fetal brain cDNA library taking VL domain as the bait. Results：7 positive clones which interact with proto-Dbl-VL domain were successfully harvested. Conclusion：This discovery lays the foundation for uncovering of the regulatory mechanism of proto-Dbl.