Effect of ERK1/2 siRNA on Transcriptional Regulation ofezrinin Esophageal Carcinoma Cells

Objective：To identify the effect of ERK1/2 siRNA on the transcriptional regulation ofezringene and elucidate the transcriptional regulatory mechanism ofezrinin human esophageal carcinoma cells. Methods：Esophageal carcinoma EC109 cells were transfected with ERK1/2 siRNA or control siRNA, and the effect of siRNA on ERK1/2 andezringenes mRNA expression was determined by real time RT-PCR. Furthermore, the role of ERK1/2 siRNA interference inezrinpromoter activity was analyzed by dual-luciferase reporter assay system. Results：Transfection of ERK1/2 siRNA to EC109 cells decreased the mRNA expression of ERK1/2 andezringenes and theezrinpromoter activity. Conclusion：ERK1/2 regulateezringene transcription in human esophageal carcinoma cells.