Tumor Inhibitory Effect of Angiostatin on C6 Glioma

Objective To investigate the tumor inhibition effect of angiostatin at different doses on the growth of C6 glioma in vivo and in vitro and to explore the feasibility that it can be a tumor angiogenesis inhibitor. Methods Angiostatin at different doses（100 ng and 1000 ng） was applied to act on the C6 glioma cell lines and tumor models in rat. The cell survival and tumor inhibition rates were calculated respectively. The SABC immunohistochemical technique was applied to detect the glioma microvessel density. Results Angiostatin at different doses had no effect on the growth of C6 cells in vitro，but it inhibited the growth of the gliorma in vivo remarkably, and the efects were at a dose-dependent pattern. The tumor inhibition rate reached 65.3％(P<0.01). Microvessel density of the glioma treated by angiostatin was lower than that of the control group（P<0.01）. Conclusion Angiostatin can inhibit the glioma angiogenesis at a dose-dependent pattern. It may be a promising basic study for angiostatin as a tumor angiogenesis inhibitor in glioma treatment.