Effect of Angiogenesis on Growth and Metastasis of Lewis Lung Carcinoma

Objective Establishing an animal model that can simulate the dynamic process of tumor progression and metastasis and to explore the important role of angiogenesis in tumor progression and metastasis by this model. Methods The Lewis lung carcinoma(LLC) was maintained by serial passage in C57BL/6 mice, to observe the dynamic process of tumor progression and metastasis of the second generation(the group of earlier stage),the eighth generation(the group of intermediate stage) and the fifteenth generation(the group of advanced stage), immunohistochemistry was used to detect the expression of HIF-1α、VEGF and MVD in tumors from each group. Results (1) With increasing numbers of passage, the tumor's growth velocity speeded up, the invasive power was gradually enhanced and the rate of pulmonary metastasis increased. (2)The expression of HIF-1α，VEGF and MVD in tumors from the group of earlier stage、intermediate stage and advanced stage were different (P<0.05).What's more, there was statistically significant correlation betweeen the expression of HIF-1α and VEGF;VEGF and MVD. Conclusion The growth velocity of LLC speeded up and the tumor′s metastasis potential was enhanced in the process of serial passage in mice,and angiogenesis played an important role in this dynamic process. As a result, tumor can keep on growing and developing pulmonary metastasis.