Effect of Integrin-mediated Signal Transduction Pathways on Apoptosis of K562 Cells

Objective To evaluate the role of integrin mediated signal transduction pathway in the apoptosis of K562 cells. Methods The percentage of apoptotic K562 cells induced by Anti-α5β1 was measured by FCM and acridine orange/ethidium bromide (AO/EB) double fluorescent dye staining, the expression levels of PI3K and survivin mRNA were measured by RT-PCR. The AKT and PI3K protein expression levels and the phosphorylation levels of these proteins were detected by Western blot method. Results Anti-α5β1 antibodies induced apoptosis of K562 cells and down-regulated the expression levels of PI3K mRNA and protein, survivin mRNA and AKT protein. The effect of Anti-α5β1 was much stronger than that of IFNα-2b. Conclusion The PI3K-AKT-survivin signal transduction pathway mediated by integrin α5β1 might contribute to the apoptosis of K562 cells.