Influence of SPRIDA with Ｍice H ₂₂ Liver Cancer Gene Expression Profiles Detected with Gene Chip Technology

Objective To study gene differential expression profiles by SPRIDA in tumor-bearing mice. To explore the potential molecular mechanism of anticancer for SPRIDA. Methods Gene expression profiles after treatment with SPRIDA in tumor-bearing mice were determined by Oligo chip technology as well as the pathway clustering and statistic analysis of single gene. Results 100 mg/kg SPRIDA treatment for 10 days caused the expression alteration of 358 genes (8.74% of total genes on the chip). Among them, 172(4.20％，172/4096) genes were up-regulated, and 186（4.54％，186/4096）genes were down-regulated. Pathway analysis showed that they belonged to apoptosis, cell cycle, natural killer cell mediated cytotoxicity, cell adhesion molecules, TGF-beta and wnt signaling pathways. Conclusion The inhibitory effect of SPRIDA on tumor growth and metastasis were made by the influence of tumor cell cycle, regulation of apoptosis and cell adhesion genes, etc.