Effects of Trichostatin A on Inhibiting Growth and Inducing Apoptosis of Thyroid Cancer Cells in Vitro

Objective To investigate the effects of Trichostatin A on inhibiting growth and induing apoptosis of thyroid cancer cell lines in vitro. Methods The cytotoxic effects of Trichostatin A on thyroid cancer cells were determined by using SRB assay. DNA content was measured by PI staining through flow cytometry. Apoptosis of thyroid cancer cells induced by Trichostatin A was identified by fluorescence microscopy with Hoechst33342/PI staining. Results After incubation of thyroid cancer cells with Trichostatin A for 48h, the inhibiting rate, with the augment of drug concentration, increased significantly（F=35.67, P＜0.01）. Trichostatin A induced apoptosis of thyroid cancer cells with Sub-G1 phase peak increasing in flow cytometry（t=6.225, P＜0.01）. Conclusion Trichostatin A possesses inhibiting proliferation and apoptosis inducing activity on thyroid cancer cells. These effects appear to be dose-dependent.