Effect of Simultaneously Blocking VEGF，hTERT and Bcl-xl Expression on Laryngeal Squamous Carcinoma

Objective To investigate the effects of inhibition VEGF，hTERT and Bcl-xl by RNA interference on laryngeal carcinoma in vitro and in vivo. Methods A recombinant plasmid containing 3 different short hairpin RNA (shRNA) segments termed pshRNA-VEGF-hTERT-Bcl-xl was used. Cells were treated with these plasmids. Cell viability was examined by using the MTT assay. The cytotoxic effect of pshRNA-VEGF-hTERT-Bcl-xl was evaluated in the subcutaneous xenograft tumors. Results pshRNA-VEGF-hTERT-Bcl-xl exhibited a potent antitumor effect on Hep-2 cells. MTT assay confirmed cytotoxic effect. The in vivo study showed tumor volume was significantly smaller in pshRNA-VEGF-hTERT-Bcl-xl treated group than that in control group. The inhibitory rate was 90.2%. Conclusion Our study demonstrated that the application of vector-based RNAi technology involved in blocking multiple targets would be a promising therapeutic modality in the gene therapy of human laryngeal cancers.