Screening and Characterization of Multipeptide Binding Specifically to Gastric Cancer Cells by Phage Peptide Library

Objective To screen and characterize multipeptides which bind specifically to gastric cancer cells by mean of screening from phage random 12 peptide library. Methods Gastric cancer cells were used as the target cells and normal cells as the absorber cells for substaction biopanning from a 12-mer peptide phage random peptide library. The stochastically selective positive phage clones were identified by using cell enzyme-linked immunosorbent assay (ELISA), immunocytochemistry, binding to nude mice tissue, and DNA sequencing. Results After 3 rounds of screening, 8 positive clones which can all bind to gastric cancer cells were obtained by ELISA, among which No.20, 24 positive clones can specifically bind gastric cancer cells but not bind to normal cells and nude mice tissue. No conserved motif was found in these peptides. Conclusion We screened Two peptides which can specifically bind to gastric cancer cells were screened from phage peptide library. This offered basic experimental data for further studying.