Expression of hk10 in Ovary Cancer Tissues

Objective To study the expression of human kallikrein 10(hk10) in ovary cancer tissues and discuss the significances of hk10 gene in the early diagonosis of ovary cancer. Methods 1.RT-PCR method was used to investigate the level of hk10 mRNA in tissue samples of 10 ovary serous cystadenocarcinoma, 10 ovary serous cystadenoma and 10 normal ovaries. 2.The expressions of hk10 protein in 60 ovary serous cystadenocarcinoma, 24 ovary serous cystadenoma and 24 normol ovary tissues was detected by immunohistochemistry. Results 1.The level of hk10 mRNA expression in ovary cancer tissues was significantly higher than in normal ovary and benign ovary tumor tissues（P<0.01）,and there was no difference between benign ovary tumor tissues and normal ovary tissues（P>0.05）.2.The positive expression rate of hk10 protein in ovary cancer tissues was significantly higher than in normal ovary tissues and benign ovary tumor tissues(P<0.01). The higher pathological grading of ovary cancer,the stronger positive expression of hk10 protein; the positive expression rate of hk10 protein was higher in Ⅱand Ⅳ ovary cancer than in Ⅰovary cancer. Conclusion The expression of hk10 mRNA and protein in ovary cancer tissues was significantly increased; There was correlation between the expression intensity of hk10 protein, pathological grading and clinical stages. hk10 may plays an important role in the study of early diagonosis for ovary cancer.