Preparation and Identification of Monoclonal Antibodies Against Human Stathmin

Objective To obtain monoclonal antibodies against stathmin for further study of the biological function of stathmin protein. Methods Balb/c mice were immunized with recomb inant stathmin,hybridoma cell lines secreting monoclonal antibodies against stathmin were screened by regular cell fusion and subcloning approach.The specificities of these monoclonal antibodies were determined by ELISA,Western blot,and immunohistochemistry. Results Two hybridoma cell lines( F001 and F002）stable insecreting specific monoclonal antibodies were successfully obtained.The subtype of the two monoclonal antibodies belong to the IgG1.Western blot analysis and immunohistochemistry showed that the two antibodies can specifically bind with stathmin antigen derived from human eucaryotic cells or tissue. Conclusion Monoclonal antibodies against stathmin with high titers and specificity have been successfully prepared,providing foundation for further study of stathmin protein and the relationship between stathmin and tumors.