Inhibitory Effects of HER2 RNAi on Proliferation of HT29 Cells

ObjectiveTo investigate the inhibitory effect of RNAi targeting HER2 on the proliferation of human colorectal cancer cells in vitro. Methods Human colorectal cancer HT-29 cells were transfected with plasmid expressing shRNA-HER2 by LipofectamineTM2000. The mRNA level and expression of HER2 in HT29 cells of each group were detected using semi-quantitive reverse transcription-polymerase chain reaction(RT-PCR) and Western blot，respectively. Inhibitory effect on proliferation of each group was measured by MTT assay. Results Plasmid mediated shRNA-HER2 can depress both transcriptional and translational level of HER2 in HT29 cells in vitro. Compared with control the proliferation of HT-29 cells was inhibited by shRNA-HER2 (P<0.01). Conclusion Plasmid mediated shRNA-HER2 can inhibit the proliferation of HT-29 cells by reducing the HER2 gene expression in vitro.