Inhibitory Effects of 5F from Pteris semiinnataL. on Proliferation of Non-small Cell Lung Cancer NCI-H460 Cells

Objective To investigate inhibitory effects of 5F from Pteris semiinnataL. on proliferation of Non-small cell lung cancer NCI-H460 cells. Methods NCI-H460 cell growth inhibition mediated by 5F was detected by MTT. PI-Hoechst double staining and TUNEL assay were used to observe cell apoptosis. Flow cytometer was used to determine the effect of 5F on the content of DNA in cells. Results 5F displayed growth inhibitory activity against NCI-H460 cells with IC₅₀ values of 21.40,4.52 and 1.02 μg/ml at the point of 24, 48 and 72 hours. 5F could induce apoptosis. NCI-H460 cells treated by 5F were arrestet in G₂/M. Conclusion In vitro 5F significantly inhibited the proliferation of NCI-H460 cells and the activity might be realized through inducing apoptosis.