Effect of CXCR4/SDF-1α in Derected Migration and Proliferation of HeLa Cells

Objective To investigate the expression of CXCR4 in HeLa cells,and evaluate the effect of CXCR4/ SDF-1α in directed migration and proliferation of HeLa cells. Methods HeLa cells were immunohistochemically stained with mouse anti-CXCR4 monoclonal antibody (mAb).In vitro invasion of HeLa cells was evaluated using Transwell Permeable Supports,in which HeLa cells with/without CXCR4 mAb pre-incubation were seeded in the upper chambers,medium containing 0～100ng/ml of SDF-1α was added to the lower compartments.For evaluating the effect of CXCR4/ SDF-1α on proliferation of cervical cancer cells,HeLa cells were cultured for 72 hours exposed to SDF-1α with and without CXCR4 mAb. Results CXCR4 was expressed on all HeLa cells.SDF-1α induced the directed migration of HeLa cells with a concentration-depended model,which was inhibited by using CXCR4 mAb.SDF-1α also stimulated the proliferation of HeLa cells mediated by CXCR4. Conclusion CXCR4/ SDF-1α participate in the directed migration and proliferation of HeLa cells.