Effect of Resistin-13-peptides on Proliferation and Apoptosis of MCF-7 Cells in vitro

Objective 　To investigate the potential effect s of resistin2132peptides on the growth and apopto2 sis in human breast carcinoma cells, MCF27. Methods 　Basing on the deduced amino2acide sequence f rom the cDNA, a peptide of human resistin molecule, consisting of 13 amino2acide (CSMEEAINERIQE) was synthesized. 32(4, 52Dimethyl222thiazolyl)2 2, 52diphenyl22 H2tet razolium bromide (MTT) assay was used to assess the proliferation effect s of resistin2132peptides. The apoptosis was investigated by the flow cy2 tometer, FCM. Results 　With the Resistin2132peptides t reatment of 50 、500 、5 000 and 10 000 ng/ ml, the inhibitory rate was 46 % to 92 %. The discrepancy between test and cont rol group s is statistically signifi2 cant ( P < 0. 01) . The apoptotic rate was 6. 7 %、6. 9 %、7. 5 % and 9. 8 % respectively. And the rate of cont rol group was 0. 6 % ( P < 0. 01) . Conclusion 　Resistin2132peptides can inhibit the proliferation of the cell proliferation and promote the apoptosis of the cells.