Expression of Beclin1 in Non-small Cell Lung Cancer and Its Clinical Significance

Objective 　To investigate the expression of autophagy related Beclin1 gene in human nonsmall2 cell lung cancer tissues. Methods 　Protein expression of Beclin1 was determined by immunofluorescence staining and Western Blot, mRNA expression were analyzed by RT2PCR. Results 　Immunofluorescence staining revealed that the levels of Beclin1 expression in lung cancer were significantly lower than those in adjacent noncancerous tissues and normal tissues, the expression rate was 8. 3 % ( P = 0. 000) . The Beclin1 mRNA expression in lung cancer 、adjacent noncancerous tissues、normal tissues were 1. 302 ±0. 074 、1. 691 ±0. 100 、1. 673 ±0. 081 ( F = 6. 6, P = 0. 002) . Beclin1 protein expression in lung cancer 、around cancer tissues、normal tissues were 3. 489 ±0. 293 、5. 306 ±0. 459 、6. 329 ±0. 580 ( F = 9. 73, P < 0. 01) . There was no dependability among the Beclin1 expression and pathology or clinic stage of lung cancer. Conclusion 　The protein and mRNA expression of Beclin1 in lung cancer were much lower than in adja2 cent noncancerous tissues and normal tissues, those differences were statistical significance, but in adja2 cent noncancerous tissues and normal tissues, the expression were proximal.