Study on the Relationship of Taxol-resistance of Ovarian Carcinoma with the Amplification of 2p22 on Chromosome and the Expression of hmsh2 Gene

Objective To investigate the difference of DNA copy number and gene expression on chromosome and their correlation with taxol-resistance of ovarian carcinoma. Methods Comparative genomic hybridization and RT-PCR technique were performed on the taxol-resistant cell lines and ovarian cancer tissues to compare the genomic alteration of chromosome and the hmsh2 gene expression. Results CGH results showed that the most interesting finding was the amplification of 2p22 in OC3/Tax300.Over-expression of hmsh2 gene in...