The lnduction of Cisplatin (CDDP)and Radiation on Telomerase activity of Human Laryngeal Neoplasms Cells and Explore the Mechanism

Objective To investigate the effects of Cisplatin(CDDP) and radiation on telomerase and cell cycle of human laryngeal squamous cells and explore the mechanism. Methods Hep 2 human laryngeal squamouscells were exposed to CDDP or 60 Co gamma radiation.FCM analysis,PCR based Telomeric Repeat Amplification Protocol(TRAP) coupled with ELISA and polyacrylamide gel eletrophoresis (PAGE)were used to estimate the change of telomerase activity and cell cycle. Results Through exposure to CDDP or irradiation,s...