Fluorescence quantitative PCR detected Infection of Epstein-Barr virus

Objective To establish a novel method for detecting infection of Epstein-Barr Virus(EBV)and to observe relation between EBV and nasopharyngeal carcinoma.Method Real time fluorescence quantitative PCR(FQ-PCR) and in situ hybridization(ISH) method were used to detect infection of EBV in 66 cases specimens of nasopharyngeal biopsy and embeded in paraffin,the results were compared with pathologic diagnosis. Results FQ-PCR detected EBV-DNA negative 52 cases,51 cases was inflammatory disease,1 cases was ade...