Loss of Heterozygosity on Chromosome 13q12-13 in Esophageal Squamous Cell Carcinoma

Objective 　To detect the status of loss of heterozygosity (LOH) on chromosome 13q1-213 in esophageal squamous cell carcinoma ( ECCC) and determine a minium loss region of ESC related tumor suppression gene ( TSG) . Methods 　By means of laser capture microdissection, eight microstatellite markers spinning chromosome bands 13q12-13 were used to examine 56 ESCC patient s by PCR2LOH, including 34 with a family history of upper gast rointestinal cancer and 22 without a family history of cancer. Results 　The total LOH f requency is 86 % (48/ 56) . One commonly deleted region was identified :was located on band 13q 12. 3-q 13. 1, between markers D13S267 and D13S219. Two microsatellite markers (D13S1242 and D13S289 ) had a higher LOH f requency in those patient s with a family history of upper gast rointestinal cancer compared to patient s without such a history ( P < 0. 05) . Conclusion 　More than one unidentified tumor suppression genes ( TSGs) are located on chromosome arm 13q that play a role in the development of ESCC.