Constructions and Identif ication of two Fusion Gene Vectors Based on PNP/ Mep-dR Suicide Gene System

Objective 　To const ruct two novel fusion suicide gene expression vectors, and detect their expression in human hepatocellular carcinoma cell line Hep G2. Methods 　Two fusion suicide gene PNP-TK and PNP-CD were generated respectively by recombinant DNA techniques. Two fusion genes were inserted into pcDNA 3. 0 vector separately to const ruct two suicide gene expression vectors, pcDNA 3. 0/PNP-TK and pcDNA 3. 0/ PNP-CD. Two recombinants were analyzed and identified by recombinant enzyme, PCR and sequencing. Then they were t ransfected into human hepatocellular carcinoma cell line Hep G2 by liposome-mediated method. The expressions of two fusion genes were detected in Hep G2 cell ine. Results 　Two fusion genes were const ructed successfully and the expression of two fusion genes were detected in human hepatocellular carcinoma cell line Hep G2. Conclusion 　Two fusion suicide gene expression vectors based on PNP/ Mep-dR system are effective and broad-spect rum vectors for human hepatocellular carcinoma gene therapy.