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LIU Shu-juan, HAN Jun-tao, XIN Xiao-yan, WU Yuan-ming, CHEN Su-min. Construction of The Eukaryotic Expression Vector pCDNA3.1-p93 and Its Expression in Ovarian Cancer Cell Line HO-8910[J]. Cancer Research on Prevention and Treatment, 2006, 33(01): 29-31. DOI: 10.3971/j.issn.1000-8578.1563
Citation: LIU Shu-juan, HAN Jun-tao, XIN Xiao-yan, WU Yuan-ming, CHEN Su-min. Construction of The Eukaryotic Expression Vector pCDNA3.1-p93 and Its Expression in Ovarian Cancer Cell Line HO-8910[J]. Cancer Research on Prevention and Treatment, 2006, 33(01): 29-31. DOI: 10.3971/j.issn.1000-8578.1563
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Construction of The Eukaryotic Expression Vector pCDNA3.1-p93 and Its Expression in Ovarian Cancer Cell Line HO-8910

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  • Objective To construct an recombinant vector consisted of pCDNA3.1 with DOC-2 cDNA(p93) and transfect it into human ovarian cancer cell line HO-8910 in order to investigate the function of DOC-2 gene on ovarian cancer cell. Methods The recombinant vector (pCDNA3.1-p93) was constructed by XhoI and its correction was confirmed by restriction pattern. Then pCDNA3.1-p93 was transfected into HO-8910 by Lipofectamin and the positive clone 8910-p93 and 8910-pCDNA3.1 were selected by G418. Finally, the expression of...
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