Screening Differential Expression of Taxol-resistance Related Gene of Ovarian Carcinnoma by cDNA Microarray

Objective To study the gene differential expression of taxol resistance cell line OC3/Tax300 of ovarian carcinnoma compared with other parent cell OC3,screen drug-resistance related gene by cDNA microarray, and discuss the reiationship between gene expression difference and drug-resistance. Methods The cDNA retro-transcribed from equal quantity mRNA derived from OC3/Tax300 and OC3 which were labeled with Cy5 and Cy3 fluorescence as probe respectively. The mixed probe were hybridized with BiostarH140S gene ex...