Characterization of Osteosarcoma Vaccine Cells Prepared by Electrical Cell Fusion

Objective Prepare the osteosarcoma vaccine by cell fusion and detect itsbiological properties. Methods The osteosarcoma vaccine was obtained by fusing UMR-106 and rat macrophages with electorporator.The DNA and surface antigen expression of fusion cells purified by magnetic cell sorting unit were assayed with flowcytometry.The colony assay of the cells and the analysis of CTL activity induced by fusion cells in vivo were also performed. Results The higher cell fusion efficiency can be obtained with electropor...